Local feeder/borer (Diptera: Agromyzidae) in Clematis [0158]

OrderDiptera
FamilyAgromyzidae[M,L,P,A]
Lower taxonPhytomyzinae: Phytomyza sp.
No. spp. involvedOne confirmed [1-C]
Feeding modeBorer in local area of stem
Host plantClematis virginiana (Ranunculaceae)
Larva from stem node of Clematis virginiana

Tunnels in current-year stem for a short distance (roughly 10-20mm) through the pith immediately adjacent to a node, then spends the rest of its time feeding in the node, causing extensive hollowing of the node. Dissecting an affected node reveals that the hollowed-out chamber inside is distinctly blackened. Externally, an affected node may discolor slightly but otherwise there seems to be little outward sign of the borer's presence, unless the larva has finished feeding and vacated the node, in which case an exit hole may be visible.

The only puparium the author has so far obtained was formed outside the stem, by a larva that emerged from plant material collected on October 11, 2023, after the plant material was held indoors for three days. The author did not observe the larva emerging from the stem, but after the puparium was found in the rearing bag, examination of the stem revealed an exit hole in one affected node that appeared freshly made. There was also a single leaf mine in the plant material, but the old age and small size of the leaf mine, along with the apparent absence of an exit slit in the leaf epidermis, strongly suggested the puparium did not come from the leaf. Details of the puparium also match larvae the author collected in 2022 from inside "black hole" chambers in stem nodes. These larvae were all significantly immature despite having been collected over the course of three months from midsummer to late summer, on July 9, July 20, August 16, and September 6, suggesting that larvae typically do not reach maturity until late September or early October -- a good match for the timing of the puparium from the 2023 October 11 collection. It is conceivable that some larvae could overwinter in the stems and emerge the following spring, but thus far, in the current study, no such overwintering larvae have been found. An adult female was reared from the 11 October 2023 puparium the following spring.

This local feeder/borer should not be confused with the agromyzid borer in the same host that tunnels for relatively long distances through the stem, primarily in the internodes, and pupates internally (see report here).

IMG#: 0158-07
An affected node on a stem of the hostplant. (Photo date: October 14, 2023)
IMG#: 0158-08
Detail, affected node; note the spot of dark discoloration on the surface of the node, just above the center of the image, a result of the larva's tunneling coming close to the epidermis.
IMG#: 0158-09
Dead larva in node. (Photo date: October 14, 2023)
IMG#: 0158-01
Another affected node, this one with a live larva inside. (Photo date: July 13, 2022)
IMG#: 0158-02
Larva in node.
IMG#: 0158-03
Larva, removed from node; inset shows anterior end of larva with crescent-shaped sclerite. (Photo date: July 13, 2022)
IMG#: 0158-04
Larva, removed from node. (Photo date: August 31, 2022)
IMG#: 0158-05
Anterior end of larva.
IMG#: 0158-06
Posterior spiracles of larva. (Photo date: August 31, 2022)
IMG#: 0158-10
Posterior spiracles of a dead larva found inside a node. (Photo date: October 14, 2023)
IMG#: 0158-21
Posterior spiracles of a dead larva found inside a node. (Photo date: August 2, 2022)
IMG#: 0158-11
Node with fresh exit hole. (Photo date: October 14, 2023)
IMG#: 0158-12
Detail, exit hole in node.
IMG#: 0158-13
Interior of node from previous two photos (0158-11 & 0158-12).
IMG#: 0158-14
Detail, interior of node.
IMG#: 0158-15
Dorsal view of very fresh puparium formed by larva that apparently emerged from the node in images 0158-11 through 0158-14. (Photo date: October 14, 2023)
IMG#: 0158-16
Lateral view, very fresh puparium.
IMG#: 0158-17
Anterior-dorsal view of anterior spiracles of puparium.
IMG#: 0158-18
Dorsal view, anterior spiracles of puparium.
IMG#: 0158-19
Posterior spiracle of puparium.
IMG#: 0158-20
The puparium from images 0158-15 through 158-19, after aging for approximately 48 hours, showing noticeable darkening. (Photo date: October 16, 2023)
IMG#: 0158-22
Reared adult female. (Photo date: March 17, 2024)
IMG#: 0158-23
Reared adult female, dorsal.
IMG#: 0158-24
Two views of the spent puparium from which the adult in images 0158-22 and 0158-23 emerged. In the upper panel, the anterior end of the puparium is to the right; in the bottom panel, the anterior end is to the left.

References

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Page created 10/16/23. Last update: 09/09/24